Despite a freshman-record from goalkeeper Jalen Tompkins on Sunday, Missouri edged Colorado 2-1 in overtime in Columbia, Mo.
Tompkins' 12 stops were the most for a CU goalkeeper since 2008 when Kirstin Radlinski had 12 against Oklahoma State in a 1-1 draw in the Big 12 Tournament semifinals.
"I thought we played pretty well," CU coach danny Sanchez said. " Missouri plays a style that we don't see a lot. They put you under a lot of pressure. I thought we were playing some of our best soccer of the year until the middle of the second half.
"They started putting more pressure on us late and there's only so much pressure you can take. It was a well-deserved win for Missouri. Over 90 minutes, they created more chances."
CU (2-2) once again came out strong in the first half, outshooting the Tigers (3-1) 10-8, but an early goal put the Buffs in a 1-0 deficit. MU's Kaitlyn Clark intercepted a pass near midfield and dribbled between several CU defenders unabated for an eventual breakaway and an easy score at 3:16.
The Buffs responded 20 minutes later at 23:44 as Sarah Kinzner bent a 25-yard shot into the upper-left corner of the net to tie the score at 1.
Missouri turned up the pressure again late in the second half, and Tompkins made three clutch saves in the final 10 minutes of regulation.
The Tigers earned a free kick from just right of the 18-yard box in the 94th minute, and Sarah Luebbert headed in the winning goal at 93:58.
CU finished the match with 15 shots to Missouri's 24.